Good Quality

This is a good read of a plasmid using a proven primer. Both were purified at the vendor's laboratory using the tools of a large biotech company. Custom sequencing data can achieve this quality when templates and primer are properly prepared, quantified, and designed. The peaks are tall, sharp, and relatively even. A background noise level is barely distinguishable. This reaction was free from salt (especially EDTA-sodium), protein, lipid, genomic DNA, carbohydrate, and alcohol contamination. Properly quantifying is key in sequencing PCR since it is not amplification as in standard PCR where theoretically only one molecule of target DNA is needed.