Rebecca and John Moores UCSD Cancer Center DNA Sequencing Shared Resource

Dear Valued Customer:

We would like to inform you that we will be permanently closing the DNA Sequencing Shared Resource Facility effective Thursday, April 25, 2013.

Please take this time to download ALL your data from http://dnalims.ucsd.edu/. All user accounts and data will be scheduled for termination on July 31, 2013.

We want to thank you for all your past business and support. We are very thankful and grateful in having you as our loyal customer. Other local and alternative sequencing vendors that are well-established via UCSD Marketplace are as follows:

Eton Biosciences: http://www.etonbio.com/
Genewiz: http://www.genewiz.com/
Retrogen: http://www.retrogen.com/

Please don't hesitate to contact us if there is anything we can do for you before 4/25/13. You have our continued good wishes in your research endeavors. Sincerely,

Oanh Hong and the Sequencing Team

Business hours are 9 AM to 5 PM, Monday through Friday.

Wondering how to reference us in a scientific paper? See our manuscript acknowledgement information

Interested in Cancer Center Membership?

Take advantage of our strand aligner and short sequence finder application.

Need help troubleshooting your sequences? Our Troubleshooting page has chromatograms to help you understand common sequencing problems.

DNA Sequencing Troubleshooting Seminars

Session 1: At the Cancer Center
Come to the Moores Cancer Center, room 2007 at 1:30pm on Thursday February 17, 2011 and learn how to troubleshoot and interpret your DNA sequencing data. Learn how to identify potential issues at every step of the process, from PCR to PCR clean-up to sequencing reactions to sequencing clean-up to electrophoresis. And best of all: learn how to solve these issues and obtain better and faster results!
Session 2: On Main Campus
Come to Skagg�s Pharmaceutical Sciences Building, room 1170 at 11:00 on Friday February 18, 2011 and learn how to troubleshoot and interpret your DNA sequencing data. Learn how to identify potential issues at every step of the process, from PCR to PCR clean-up to sequencing reactions to sequencing clean-up to electrophoresis. And best of all: learn how to solve these issues and obtain better and faster results!

Holiday Schedule - DNA Sequencing will be closed on the following dates:

2011:

Presidents Day, Feb 21
Cesar Chavez Day, Mar 25
Memorial Day, May 30
Independence Day, Jul 4
Labor Day, Sept 5
Veterans Day, Nov11
Thanksgiving Days, Nov 24 and 25
Christmas and New Year's Day University Shut down: Dec 21-Jan 2--->ORDERS RECEIVED by 12/20 at 12noon will be returned the same day. Anything received after 12 noon will be processed when we resume business on 1/3/12.

2012:

Martin Luther King Jr. Day, Jan 16
Presidents Day, Feb 20
Cesar Chavez Day, Mar 30
Memorial Day, May 28
Independence Day, Jul 4
Labor Day, Sept 3
Veterans Day, Nov 12
Thanksgiving Days, Nov 22 and 23
Christmas and New Year's Day University Shut down: Dec 21-Jan 1--->ORDERS RECEIVED by 12/20 at 12noon will be returned the same day. Anything received after 12 noon will be processed when we resume business on 1/2/12.

Now Offering Extraction Service (9/30/09)

View service details at our Extraction page.

Relaxed Sequencing Concentration Requirements! (4/20/08)

Plasmid template concentrations of 150-400ng per microliter are now accepted!

Recommended free software: ABI Sequence and Genotyping file viewers (4/11/08)

Sequence Scanner:
View your analyzed and raw data simultaneously along with your peak quality scores! Tabbed browsing and lots of features make this program very useful.
Peak Scanner:
View your fragment analysis files (.fsa). This is one of the only freeware genotyping viewers available! Sizecalls only, no programmable allele calls.

New Stock Primers (3/12/08)

We have added several new primers to our stock primers list. New offerings include EGFPn, EGFPc, GFPf, CMV, SV40, and an improved BGHr.