Chromatogram Tips

• Chromatogram quality should always be considered before using a sequence.
• Multiple sequencing problems may occur simultaneously.
• Black G peaks are artificially depressed when flanking green A peaks.
• The first 20 bases off of the 3'end of the primer do not make it past purification.
• The first 30 bases of a chromatogram can not be trusted. Mass differences between the bases weigh more heavily at these short lengths.